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Objective To develope a realtime system for ECG signals acquisition,amplification and network transmission.Methods The raw noisy ECG signals underwent gain amplification,denoising and filtering by a system developed by virtual instrument and LabVIEW,and network component programming was carried out based on TCP/IP.Then the processed ECG signals were transmitted to remote terminals with dual communication model.Results The system behaved well in easy operation,high reliability and man-machine interface,and could be used to realize remote realtime transmission and browsing of ECG signals between the hospital and medical communities.Conclusion The system may be a choice for ECG signals remote acquisition and transmission,and provides references for the development of telemedicine software.
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OBJECTIVE To implement the automatic management of medical equipment.METHODS Based on the platform of net,three layers of C/S were applied with database technology to realize one-time importation of the basic information and current information,it made the information function of quantity evaluation,quantity determination,schedule formation,automatic alarm,apparatus distribution list,statistical inquiry,collection analysis,and information feedback coming to true.RESULTS By using one year,the application of management information system of medical equipment could meet the requirement in scientific and standardized supply,and standard,application and management of medical equipment.CONCLUSIONS It can be able to improve the system efficiency,reduce the labor and mistakes,and also achieve the aim that cannot be done by manual labor.
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OBJECTIVE To recombine J chain gene with HNP-1 into a new germicidal(molecule) J-HNP-1,which can connect with pIgR by J chain,so that by using pIgR as a "bridge" the J-HNP-1(germicidal) peptide can be transported into the epithelial cell of mucous membrane to kill the intracellular microorganisms,then the recombinant is inserted into the mammalian expression system.METHODS The J chain and HNP-1 cDNA were amplified from the plasmids respectively by PCR,then the two cDNA fragments were recombined into J-HNP-1 by recombinant PCR.The J-HNP-1 cDNA fragment was inserted into the(mammalian) expression vector pcDNA3.1(-)/Myc-HisC.RESULTS The J-HNP-1 recombinant was obtained by(connection) of J chain and HNP-1 cDNA by PCR.The recombinant J-HNP-1 cDNA was 786bp.CONCLUSIONS The recombinant J-HNP-1 cDNA and the construction of expression vector are the basis for the new bactericidal peptide production.
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OBJECTIVE To reconstruct the HNP-1 into the J-HNP-1 with a J chain,and explore to set up a mammalian cell expression system which can express and secret J-HNP-1,so that the products could be examined and purified conveniently. METHODS The J-HNP-1 cDNA fragments were produced by recombinant PCR.Then the J-HNP-1 was inserted into the mammalian expression vector pcDNA3.1(-)/Myc-His which had the double marks Myc and 6?His.The recombinant vector rpcDNA3.1(-)/Myc-His /J-HNP-1 was transfected into the COS-7 cell.The J-HNP-1 expression was analyzed at the mRNA and protein level.The germicidal activity of cell culture supernatant and cellular solution protein was assayed. RESULTS By the use of RT-PCR with special primers,a band of 786bp was amplified from COS-7 cells transfected by this recombinant plasmid.Western blot analysis with specific anti-histidines antibody revealed that the cell culture supernatant and cellular solution protein of COS-7 cells transfected by rpcDNA3.1(-)/Myc-His /J-HNP-1 had a strong band with relative molecular mass of about 24?10~3.Antibacterial activity assay showed that obvious bacterial inhibition occurred in both lysate and supernatant of COS-7 cells transfected by rpcDNA3.1(-)/Myc-His /J-HNP-1. CONCLUSIONS The J-HNP-1 recombinant is obtained and inserted into the mammalian expression vector then to be expressed in vitro.The expression product is found to have the anti-bacterial effect in vitro.
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Objective To evaluate the performance parameters of the PET instrument, which has been used for years in our hospital, assuring the stability of image quality and performance parameters. Methods NEMA NU 2-1994 Standards was used as reference for performance measurement of PET, which were published by National Electrical Manufacturers Association(NEMA). The NEMA standard cylinder model, 18F radiation source, and 18F and 68Ge columniform radiation source were used. Results Projects for performance measurement and daily quality control were drawn up. Conclusion This project provides a reference and methods for the performance measurement of PET instrument and daily quality control. It better ensures the stability of image quality and performance parameters of the PET instrument.
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With more and more equipments installed,the quality of power in hospital becomes worse and worse,which seriously threatens the safety of equipments.To assure medical devices running stably and improve the quality of power supply,we adopted the method of brunch power supply and power net purifying instruments in improving the condition of equipments switched on power and monitoring power quality,so that the supply quality in hospital was improved,as well as equipments trouble rate was decreased.
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The development of orthopedic fixing materials is a most basic and active aspect in orthopedic injury treatment.Their invention and application of early manual splints,gypsum bangs,hot-shaped splints,polymeric bangs and so on,greatly promoted orthopedic fixing performance and varied the strategies of orthopedic injury treatment.By repeated experiments and clinical trials,we developed a new orthopedic fixing material using polymeric materials.Convenient to operate,easy to remove,auto-shaped,X ray transmittable,water proof and durable,it not only satisfies the needs of fixing different parts of the body,but also remedies the defects of earlier fixing materials,and therefore has gained an extensive application as the best fixing material for the treatment of clinical and battle-field bone fractures.Further studies on it will surely better its properties,reduce its cost and lead it to a wider application.
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Objective To improve hospital power quality and guarantee the stability of medical equipment. Methods Leg of circuit is taken for power supply. Refining plant is equipped for electric barbed wire. Conditions are improved for equipment access. Power quality monitoring is strengthened. Results Power quality is effectively improved while the failure rate decreases obviously. Conclusion It is essential to improve power quality for high medical equipment quality.
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Objective To enhance the rapid response capability of urgent medical unit to satisfy the requirements of natural and manmade disasters for medical service.Methods Modularization principle and system approach were applied.Results Modularized design and management of urgent medical equipments were realized.Conclusion It's very important to realize modularized management of urgent medical equipments.
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Objective To design a handhold emergency suction apparatus, in order to meet the needs of clinical therapy, field and on-site emergency treatment. Methods The apparatus was divided into such components as handle, grab handle, L tie, piston, waste liquid tank and its cap, and the junction between the parts, and then the optimum design of the components were performed. Results Through the optimum design, the apparatus was improved from the aspects of portability, easy-to-use and reliability. Conclusion It is proved by practice that the apparatus also can reduce the labor intensity of healthcare staff, and definitely satisfy the clinical rescue and the treatment needs.
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Objective To develop a medical equipment management information system in CAPF so as to improve current medical equipment management and maintenance level.Methods Based on a thorough research for current medical equipment management model in CAPF,the platform of NET was utilized to develop the software.The connection,combination and operation among all medical equipment management and maintenance work were realized by hardware construction at last.Results The management information system not only met the requirement in management of medical equipment,but also supplied a data basis to directors.Conclusion The management information system provides a powerful tool for the scientific and standard management of medical equipment in CAPF.